According to the rail operator, services will be “retimed” due to railway engineering improvements.
The rail line through Winchburgh Tunnel, near Linlithgow, will be closed from June 13 until July 27 as Scotrail prepares for the electrification of the route.
As a result of the Tunnel closure and the temporary timetable in place during this work, some trains on the Glenrothes line will depart at different times and may take longer to allow Alloa, Dunblane and Stirling services to use the same route.
Meanwhile, queuing systems will be in place at Edinburgh Waverley and Haymarket stations.
A ScotRail spokeswoman said: “We are advising commuters to set off earlier than normal as some journeys may take longer. Customers should check our website for times before travelling.”
The six-week long closure of Winchburgh Tunnel is part of the Scottish Government-funded, £742 million Edinburgh Glasgow Improvement Programme (EGIP) which will electrify the Edinburgh-Glasgow Queen Street line by 2016 – allowing the introduction of faster, greener and quieter electric services on the route.
As part of the 44-day project, engineers will be lowering and relaying the two lines of track through the 330m tunnel and installing equipment to carry the overhead power lines needed for the electrification of the railway.